Obtaining Multiprotein Antigens Of Brucella And Study Of Their Immunoreactivity

Rose-Bengal test, complement fixation test and the agglutination are mainly used for diagnosis of animal brucellosis. These tests characterized by low sensitivity specificity, which is one main reasons effectiveness measures aimed at eradicating The use modern highly sensitive serological requires availability antigens specific to Brucella spp. aim study was obtain multiproteins pathogen recombinant DNA technology their antigenic properties. In course study, three types were obtained, constructed from diagnostically important peptides that form B.abortus B.melitensis outer membrane proteins. All target products synthesized producer strain in a authentic natural proteins, showed immunogenicity mouse model. Antibodies produced against single proteins pathogen's cell wall. data obtained indicate need continue studies determine possibility using as an antigen enzyme-linked immunosorbent assay detect anti-Brucella antibodies.